The Effects of Intra-Ductal Mammary Administration of Orexin-A Antagonist (SB-334867) on the PPARγ and SREBP1c Gene Expression and Serum Adiponectin Levels in Lactating Rats

Introduction: Orexin A and adiponectin are involved in controlling metabolism energy distribution the body during lactation. Lactation is a process with high demand for lipid synthesis. Peroxisome proliferator-activated receptor gamma (PPARγ) sterol regulatory element-binding protein 1 (SREBP1c) two transcription factors that regulate milk The goal of this study was to assess effects mammary administration orexin antagonist on expression PPARγ SREBP1c genes as well serum levels lactating rats. Methods: (SB-334867) injected intraductal into glands female rats at three doses 1, 2 4 μg/kg BW solvent volume 50 μL. gene expressions were measured using real-time polymerase chain reaction (PCR), along ELISA. Results: results quantitative RT-PCR showed significant decrease relative compared control group. rate reduction dose µg/kg SB greater than SB-334867. significantly decreased group group.Conclusion: These indicated intra-mammary orexin-A fat synthesis, accompanying by level.